Clinical Microbiology

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Last 4 digits of SSN *

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Proficiency Scale

1 = No Experience

2 = Need Training

3 = Able to perform with supervision

4 = Able to perform independently

Orientation and Lab Safety

Review the laboratory's fire safety plan. *

Locate Personal Protective equipment and MSDS. *

Understand Universal Precautions for microbiology. *

Labeling & Specimen ID

Label specimens according to institutional policies. *

Specimen Set Up & Incubation

Select proper media for specimens including plated media and broth media. *

Understand specimen collection & rejection criteria. *

Incubate specimens properly. *

Inoculation

Demonstrate plate streaking for isolation & quantitative streaking for urines. *

Quality Control

Perform quality control procedures in accordance with institutional policies for new media, reagents, and stock culture organisms. *

Understand documentation & actions taken when results are not in acceptable limits. *

Gram Staining

Practice performing Gram stains until proficient. *

Evaluate grams stains, including sputum samples, wounds, genital samples, and positive blood cultures until proficient. *

Evaluation of Primary Cultures

Evaluate cultures to recognize what is normal flora and what is significant. *

Evaluate throat cultures & select next course of action. *

Evaluate urine cultures to decide when susceptibility testing is warranted. *

Evaluate vaginal cultures to recognize what is normal flora and what is significant. *

Evaluate stool cultures to recognize what is normal and what to process further. *

Evaluate body fluid cultures for pathogens. *

Evaluate wound cultures to recognize what is significant -select next course of action. *

Evaluate respiratory cultures, including sputum cultures. Recognize normal resp. flora & significant pathogens. *

Blood Culture Processing

Demonstrate procedure for processing positive blood cultures including subcultures, Gram stains, and proper reporting of results. *

Primary ID of Organisms

Recognize Streptococcus species & perform tests to classify them according to the Lancefield classification scheme. *

Recognize & perform tests to identify Staphylococcus species. *

Identify coagulase negative Staphylococcus species. *

Recognize and identify Haemophilus species. *

Recognize and identify Neisseria species. *

Recognize and perform biochemical tests to ID GNBs, including lactose fermenters, nonfermenters, and other miscellaneous GNBs. *

Recognize and identify Campylobacter species. *

Interpretation and Acceptance of Results

Discuss recording, reporting, and documenting results. *

Discuss which organisms are reportable to the State Health Department. *

Serological Testing

Perform Rapid test for Group A Streptococcus, include proper labeling, QC, and reporting. *

Affective Objectives - demonstrates honesty by:

Maintaining strict patient confidentiality. *

Accepting control values only when within acceptable limits. *

Performing and documenting daily & weekly maintenance procedures, preventative maintenance, temperature checks, etc. *

Completing all procedures in adherence to laboratory SOPs, taking no shortcuts or unauthorized modifications of procedure. *

Personal Interactive Skills - demonstrates proper professional behavior by:

Working with co-workers in a positive manner, promoting productive workflow. *

Refraining from making statements or actions that represent sexual, ethnic, racial, or homophobic harassment. *

Willingly and consistently using appropriate personal safety devices when handling caustic, infectious, or hazardous materials. *

Completing all required tasks and remaining in the work area when scheduled. *

Being punctual whenever scheduled. *

Adhering to current dress and appearance in the laboratory setting. *

Cleaning the work area when leaving the laboratory, returning supplies to appropriate storage location, & disinfecting all work areas used by the student. *

Professional Responsibility - demonstrates appropriate professional affective behavior by:

Correctly reporting all patient test values, as well as recognizing and correctly reporting all patient critical test values. *

Resolving discrepancies in specimen labeling, handling, or collection before reporting patient results. *

Clinical Microbiology

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